产品详情
  • 产品名称:HelaNuclearExtract

  • 产品型号:
  • 产品厂商:KamiyaBiomedical
  • 产品文档:
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简单介绍:
HelaNuclearExtract
详情介绍:
Background Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
Application Notes Western blot: Use at 10-20 µg/lane HDAC. Fluorometric Assay: Use at 10 µg/assay HDAC. Colorometric Assay: Use at 50 µg/assay
Restrictions For Research Use only
Format Liquid
Buffer 200 µL of HeLa nuclear extract at 5 mg/mL in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl2, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF.
Storage -80 °C