产品详情
  • 产品名称:HumanQuantibodyKiloplex

  • 产品型号:
  • 产品厂商:RayBiotech
  • 产品文档:
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简单介绍:
HumanQuantibodyKiloplex
详情介绍:
Purpose Quantibody? Human KiloPlex Array. A combination of 25 nonoverlapping arrays. Detects 1000 Human Biomarkers. Suitable for all liquid sample types.
Sample Type Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell Lysate, Tissue Lysate
Analytical Method Quantitative
Detection Method Fluorometric
Specificity 17-beta-HSD1 (HSD17B1), 2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 5'-Nucleotidase (CD73), 6Ckine (CCL21), ACE, ACE-2, Activin A, Activin R1A (ALK-2), Activin R1B (ALK-4), Activin R2A, Activin R2B (ACVR2B), ADA, ADAM-12, ADAM-22, ADAM23, ADAM-8, ADAM-9, ADAMTS-13, ADAMTSL-1, Adiponectin (ACRP30), Adipsin (Complement Factor D), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), Aggrecan, AgRP, AIF, AKR1C4, Albumin, ALCAM (CD166), ALK-1, ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, AmiGO, AmiGO2, Aminopeptidase LRAP (ERAP2), Aminopeptidase P2 (XPNPEP2), Amnionless, Amphiregulin, AMSH (STAMBP), Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, ANGPTL7, Annexin A5 (Annexin V), APRIL, Arginase 1, ARNT (HIF-1 beta), Artemin, Arylsulfatase A (ARSA), Arylsulfatase B (ARSB), ASAH2 (Neutral Ceramidase), ASAHL, AXL, B3GNT2, B4GalT1, B7-H1 (CD274), B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, BAI1, BAMBI (NMA), BATF3, BCAM, BCL-10, BCL-2, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), Beta-glucuronidase (GUSB), beta-NGF, bFGF, BID, Biglycan, BLAME (SLAMF8), BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA (ALK-3), BMPR-II, BOC (Brother of CDO), Bora, Brevican, Brorin (VWC2), C1qTNF4, C1qTNF9, C5a, CA12, CA125, CA13, CA14, CA15-3, CA19-9, CA2, CA4, CA5A, CA5B, CA6, CA72-4, CA8, CA9, Cadherin-11, Cadherin-13, Cadherin-17, Cadherin-4, Cadherin-6 (KCAD), Calcitonin, Calreticulin, Calreticulin-2, Calsyntenin-1 (Alcadein-alpha), CANT1 (SCAN-1), Carboxylesterase 1 (CES1), Carboxylesterase 2 (CES2), Carboxypeptidase A2 (CPA2), Carboxypeptidase B1 (CPB1), Carboxypeptidase E (CPE), Cardiotrophin-1 (CT-1), Caspase-7, Caspase-8, Caspr2, Cathepsin B (Pro-Cathepsin B), Cathepsin E, Cathepsin H, Cathepsin L, Cathepsin S, Cathepsin V (Cathepsin L2), Caveolin-2, CCL28 (MEC), CD109, CD14, CD147 (EMMPRIN), CD155 (PVR), CD163, CD2, CD200, CD200 R1, CD229 (SLAMF3), CD23, CD26 (DPPIV), CD27 (TNFRSF7), CD27 Ligand (TNFSF7), CD28, CD30 (TNFRSF8), CD300a (LMIR1), CD300c (CLM-6), CD300e (LMIR6), CD300f (CLM-1/IREM-1), CD314, CD320 (TCblR/8D6A), CD34, CD36 (SR-B3), CD39L2 (ENTPD6), CD39L3, CD39L4 (ENTPD5), CD4, CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD42b (GPIb-alpha), CD48 (SLAMF2), CD5, CD51 (Integrin alpha-V), CD58 (LFA-3), CD6, CD69, CD7, CD80 (B7-1), CD83, CD84 (SLAMF5), CD86 (B7-2), CD97, CD99, CD99-L2, CDC25B, CDNF, CDO, CEA (CEACAM-5), CEA Variant (CEACAM-5 Variant), CEACAM-1, CEACAM-3 (CD66d), Cerberus 1, Chemerin, CHI3L1, CHL-1 (L1CAM-2), CHMP2B, CHST1 (GST-1), CHST2, CHST3, CHST4 (GST-3), cIAP-1 (BIRC2/hIAP-2), cIAP-2, CILP-1, Ck beta 8-1 (CCL23/MPIF-1), CLEC-1, CLEC10A (CD301), CLEC-2, CLEC9A, Clusterin, CMG-2 (ANTXR2), c-Myc (MYC), CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor/Thromboplastin), Coagulation Factor VII, Coagulation Factor X (CF10), Coagulation Factor XIV (Protein C), Cochlin (COCH-5B2), Collectin-12 (CL-P1), Common beta Chain, Complement MASP3, COMT, Contactin-1, Contactin-2 (TAG1), Contactin-3 (BIG-1), Contactin-5, Cortactin, CREG, CRELD2, CRIM1, Cripto-1, CRISP-2, CrkL, CRP (C-Reactive Protein), CRTAC1, CRTAM, CTACK (CCL27), CTLA-4 (CD152), CTRC (Chymotrypsin-C/Caldecrin), CXADR, CXCL14 (BRAK), CXCL16, Cyclophilin A, Cyr61 (CCN1), Cystatin A, Cystatin B, Cystatin C, Cystatin D, Cystatin E/M, Cystatin S, Cystatin SA (Cystatin 2), Cystatin SN, Cytokeratin 18 (KRT18), Cytokeratin 19 (KRT19), Cytokeratin 8 (KRT8), DAN, DAPP1, DBH, DcR3, DC-SIGNR (CD299), DDR1, Decorin, Dectin-1 (CLEC7A), Dectin-2 (CLEC6A), Desmin, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoglein-3, DFF45 (ICAD), DKK-1, DKK-3, DKK-4, DLL1, DLL4, DNAM-1 (CD226), DNMT3A, Dopa Decarboxylase (DDC), DPPII (QPP/DPP7), DR3 (TNFRSF25), DR6 (TNFRSF21), DRAK1, Draxin (Neucrin), DSCAM, DSPG3, Dtk, Dynactin subunit 1 (DCTN1), E-Cadherin, EDA-A2, EDAR, EDIL3, EGF, EGFR, EG-VEGF (PK1), EMR2, ENA-78 (CXCL5), Endocan, Endoglin (CD105), Endoglycan (PODXL2), eNOS (NOS3), ENPP-2 (Autotaxin), ENPP-7 (Alk-Smase), Enteropeptidase, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), EphA1, EphA2, EphB2, EphB3, EphB4, EphB6, Ephrin-A4, Ephrin-B3, Epimorphin (Syntaxin 2), Epiregulin, ErbB2, ErbB3, ErbB4 (HER4), Erythropoietin, Erythropoietin R, ESAM, E-Selectin, Exostosin-like 3 (EXTL3), FABP1, FABP2, FABP4 (A-FABP), FABP6, FABP8 (M-FABP), FAM3C, FAP, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc epsilon RI, Fc gamma RIIB/C (CD32B/C), FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), FCRL3 (FcRH3), FCRL5 (FcRH5), FCRLB (FCRY), Ferritin, Fetuin A, FGF R5 (FGFRL1), FGF-12, FGF-16, FGF-17, FGF-19, FGF-20, FGF-21, FGF-23, FGF-3, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-9, Fgr, Ficolin-1, FKBP51, FLRG, FLRT1, FLRT2, Flt-3 (Flk-2), Flt-3 Ligand, FOLH1 (PSMA), Follistatin, Follistatin-like 1, FOLR1, FOLR2, FosB (G0S3), Fractalkine (CX3CL1), FRS2, FSH, FUCA1, Fucosyltransferase 8 (FUT8), Furin, Galanin, Galectin-1, Galectin-2, Galectin-3, Galectin-4, Galectin-7, Galectin-8, Galectin-9, GALNT10, GALNT2, GALNT3, Gas 1, Gas 6, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), Gastric Intrinsic Factor, Gastrokine-1 (GKN1), GATA4, GATA-5, GBA3, GCP-2 (CXCL6), GCSF, GCSF R (CD114), GDF-11 (BMP-11), GDF-15, GDF-3, GDF-8 (Myostatin), GDF-9, GDNF, GFAP, GFR alpha-1 (GDNF R), GFR alpha-2, GITR (TNFRSF18), GITR Ligand (TNFSF18), GLI-3, GLP-1, Glyoxalase II, Glypican 1, Glypican 2, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, GOLM1 (GP73), gp130 (sgp130), GPR111, GPR115, GPR56, GPV (CD42d), GPVI, Granulysin (NKG5/LAG-2), Granzyme A, Granzyme B, Granzyme H, GRAP2, GRK5, GRO alpha (CXCL1), GRO alpha/beta/gamma, Growth Hormone, Growth Hormone R, GSTM1, HAI-1, HAI-2, HAPLN1, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), hCG beta, HE4 (WFDC2), HepaCAM, Hepsin, HGF, HGFR, HIF-1 alpha, HS3ST1, HS3ST3B1, HS3ST4, HSP32 (HO-1/HMOX1), HTRA2, HVEM (TNFRSF14), Hydroxyacid oxidase 1 (HAO1), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), ICOS, IDO, IFN-alpha / beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGSF3, IGSF4B (SynCAM3/CADM3), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R5 (IL-18 R alpha), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18, IL-18 BP alpha, IL-18 R beta (AcPL), IL-19, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 R alpha 1, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-27 R alpha, IL-28 R alpha (IFN-lambda R1), IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-31 RA, IL-32 alpha, IL-33 (IL-1 F11), IL-34, IL-36 alpha (IL-1 F6), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-36 Ra (IL-1 F5), IL-37 (IL-1 F7), IL-38 (IL-1 F10), IL-4, IL-4 R alpha, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha (CD127), IL-8 (CXCL8), IL-9, ILT-2 (LIR-1/CD85j), ILT-4 (LIR-2/CD85d), Inhibin A, Insulin, Insulin R (CD220), Integrin alpha 1 (CD49a), Integrin alpha 2 (CD49b), Integrin alpha 5, Integrin alpha-M (CD11b), IP-10 (CXCL10), I-TAC (CXCL11), Jagged 1, Jagged 2, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), JAM-C (CD323), Kallikrein 1, Kallikrein 11, Kallikrein 12, Kallikrein 14, Kallikrein 5, Kallikrein 7, Kell (CD238), KIR2DL3 (CD158b2), KIRREL3 (NEPH2), KLF4, Kremen-2, Kynureninase, LAG-3, LAIR1, LAIR2, Laminin alpha 4, LAMP (LSAMP), LAMP1 (CD107a), LAMP2 (CD107b), Langerin (CD207), LAP/TGF beta 1 (TGF beta 1), Layilin, LDL R, LEDGF, Legumain, Leptin, Leptin R, LIF, LIF R alpha, LIGHT (TNFSF14), LILRB4 (CD85k/ILT-3), LIMPII, Lin-28A, Lipocalin-1, Lipocalin-2 (NGAL), LOX-1, LOXL2, LRIG1, LRIG3, LRP-6, LRRC4, LRRTM3, LRRTM4, LSECtin (CLEC4G), L-Selectin (CD62L), Lumican, Luteinizing hormone, Lymphotactin (XCL1), Lymphotoxin beta R (TNF RIII), LYVE-1, MAP1D, Marapsin, Matrilin-2, Matrilin-3, Matriptase (ST14), MBL, MCEMP1, Mcl-1 (Bcl2L3), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MDGA1, MDGA2 (MAMDC1), MDL-1 (CLEC5A), MDM2, MEF2C, MEPE (OF45), Meprin alpha, Meprin beta (MEP1B), Mer, Mesothelin, METAP2, MFRP, MIA, MICA, MICB, Midkine, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MIS RII (AMHR2), MMP-1, MMP-10, MMP-12, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MOG, MPIF-1 (CCL23), MSP alpha/beta, MSP Receptor (Ron), NAP-2 (PPBP/CXCL7), N-Cadherin (Cadherin-2), NCAM-1 (CD56), NCK1, Nectin-1, Nectin-2 (CD112), Nectin-3, Nectin-4, Neprilysin, Neprilysin-2 (MMEL1), Nesfatin-1, Nestin (MMEL1), Netrin-4, Neudesin, Neurexin-3 beta (NRXN3), Neurocan, Neuroglycan C (CSPG5), Neurogranin, Neuroligin 2, Neuropilin-2, Neurturin, NG2 (MCSP), NGFR (TNFRSF16), Nidogen-1, Nidogen-2, NKp30, NKp44, NKp46 (NCR1/CD335), NKp80 (KLRF1), Nogo Receptor (Reticulon-4 Receptor), Nogo-A (Reticulon 4A), Norrin, Notch-1, Notch-3, NOV (CCN3), NPDC-1, NPTXR, NQO-1, NrCAM, Nrf2, NRG1-alpha (HRG1-alpha), NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, NTAL, NTB-A (SLAMF6), NUDT5, Numb, NUP85 (FROUNT), Olfactomedin-2 (Noelin-2), Olig2, Omgp, Oncostatin M, Oncostatin M R beta (IL-31 R beta), OSCAR, Osteoactivin (GPNMB), Osteoadherin (Osteomodulin), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27/Kip1 (CDKN1B), p53, p63 (TP73L/KET), PAI-1, Pancreatic Polypeptide (PPY/PP), PAPP-A, PAPP-A Heterotetramic (htPAPP-A), PAPP-A2, PAR1, PARC (CCL18), Pax3, P-Cadherin, PCK1 (PEPCK-C), PCSK2, PD-1, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PD-L2 (B7-DC), PDX-1 (IPF1), PEAR1, PECAM-1, Pentraxin-3 (TSG-14), Pepsinogen 1, Pepsinogen 2, Periostin, Peroxiredoxin-2 (PRDX2), Peroxiredoxin-4 (PRDX4), Persephin, PGRPs, PILR-alpha, Platelet Factor 4 (CXCL4), Plexin A4, Plexin B3, Plexin D1, PLGF, PlGF-2, Podocalyxin, Podoplanin, POGLUT1, PON1, Pref-1 (DLK-1/FA1), PRELP, Presenilin 1, Procalcitonin, Progranulin, ProGRP, Prolactin, Prolactin R, Prostasin, PSA-free, PSA-total, PSMA1, PTH, PTH1R, PTP1B, PU.1 (Spi-1), PYY, RAGE, RalA, RANK (TNFRSF11A), RANTES (CCL5), RBP4, RCOR1 (CoREST), Reg1B, Reg3A, Reg4, RELT (TNFRSF19L), Renin, Resistin, Ret, RGM-A, RGM-B, RGM-C (Hemojuvelin), ROBO2, ROBO3, ROBO4, ROR1, ROR2, R-Spondin 2, Ryk, S100 A1, S100 A13, S100 A8, SALM4, SCCA2 (Serpin B4), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Semaphorin 4C, Semaphorin 4D, Semaphorin 4G, Semaphorin 6A, Semaphorin 6B, Semaphorin 6C, Semaphorin 6D, Semaphorin 7A, Serpin B6, Serpin B8, SERPINA4, SERPINA5 (Protein C Inhibitor), SERPINF1 (PEDF), sFRP-3, SH2D1A, SHP-1, SIGIRR, Siglec-1 (CD169), Siglec-10, Siglec-11, Siglec-2, Siglec-5 (CD170), Siglec-6, Siglec-7 (CD328), Siglec-9, SIRP alpha, SIRP-gamma (CD172g), Sirtuin 1, Sirtuin 2, Sirtuin 5, SLAM (CD150), SLITRK5, Smad4, SMPD1, Sonic Hedgehog N-Terminal (Shh-N), SorCS1, SorCS2, SorCS3, Sortilin, SOST, SOX15, SOX2, SOX7, SOX9, SP-D, SPHK1, Spinesin, SPINK1, SR-AI (MSR), SREC-I (SCARF1), SREC-II (SCARF2), ST3GAL1, ST6GAL1, ST8SIA1, Stabilin-2, SULT2A1, Syndecan-1, Syndecan-3, Syndecan-4, Syntaxin 4, Syntaxin 6, TACE, TACI (TNFRSF13B), TAFA1 (FAM19A1), TAFA2 (FAM19A2), TAFA5 (FAM19A5), TARC (CCL17), TAZ (WWTR1), TC-PTP, TECK (CCL25), Tenascin R, Testican 2 (SPOCK2), TFF3 (Trefoil Factor 3), TFPI, TFPI-2, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, THAP11, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin 1, Thrombospondin 2, Thrombospondin 5 (COMP), THSD1, Thyroglobulin, Thyroid Peroxidase, Tie-1, Tie-2, TIM-1 (KIM-1), TIM-3 (KIM-3), TIMP-1, TIMP-2, TIMP-4, TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TPST2, TRACP (PAP/ACP5), TRAIL (TNFSF10), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE (TNFSF11), Transcobalamin II, Transferrin, Transglutaminase 3 (TGM3), Transglutaminase 4 (TGM4), Trappin-2, TREM-1, TREM-2, TREML1 (TLT-1), Tripeptidyl-peptidase I (TPP1), TrkA, TRKC, TROP1 (EpCAM), TROP-2, Troponin C, Troponin I, TROY (TNFRSF19), Trypsin 1 (PRSS1), Trypsin 3 (PRSS3), TSH, TSLP, TSLP R, Tsukushi (TSK), TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, UCH-L3, ULBP-1, ULBP-2 (ALCAN-alpha/N2DL2), ULBP-3, ULBP-4 (RAET1E), UNC5H3, UNC5H4, uPA, uPAR, Uromodulin, VAMP-1, VAMP-2, VAP-A, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, Visfatin, vWF-A2, WIF-1, WISP-1 (CCN4), Wnt-4, XEDAR, XIAP (BIRC4/IAP-3)
Characteristics
  • Running an array is like running dozens of ELISAs simultaneously.
  • Quantibody arrays are stunningly simple to use, read, and analyze.
  • Each panel can quantify up to 40 different biomarkers simultaneously, and individual panels can be multiplexed to quantify as many as 660 different biomarkers at one time.
  • The entire process can be completed in just 4-6 hours.
  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 μL or less
  • Well-suited for high throughput assays

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 μL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation
Components Glass Chip with antibody arrays
Sample Diluent
Lyophilized protein standard mix
Detection antibody cocktail
Streptavidin-Fluorescent dye
Wash buffer
Material not included Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
Application Notes Completely cover array area with sample or buffer during incubation. Avoid foaming during incubation steps. Perform all incubation and wash steps under gentle rocking or rotation. Cover the incubation chamber with adhesive film during incubation, particularly when incubation is more than 2 hours or <70?μl 6="" 7="" 10="" 13="" of="" sample="" or="" reagent="" is="" used.="" several="" incubation="" steps="" such="" as="" step="" (blocking),="" (sample="" incubation),="" (detection="" antibody="" (cy3="" equivalent="" dyestreptavidin="" incubation)="" may="" be="" done="" overnight="" at="" 4?°c.="" please="" make="" sure="" to="" cover="" the="" chamber="" tightly="" prevent="" evaporation.="" <="" td="">
Comment

The Quantibody arrays are quantitative multiplex ELISA arrays featuring fluorescent detection. The antibodies are spotted on glass slide solid supports and require a laser scanner for data collection. Cytokine standards are provided with the array for calculation of target protein concentrations.
All Quantibody arrays feature the sandwich immunoassay principle, utilizing an immobilized capture antibody along with a corresponding biotinylated detection antibody.

Sample Volume 100 μL
Plate Glass Slide
Protocol
  1. Each Quantibody array starts with a single glass microscope slide, which acts as a support for the array. Slides are segmented using a rubber gasket. Up to 8 samples may assayed using a single slide.
  2. Antibodies against a variety of different antigens (up to 40 biomarkers per slide) are printed onto the glass slide. Replicates are included, saving you both time and precious sample volume.
  3. The end-user adds either known concentration standards (included) or aqueous sample to each well on the slide. Antibodies on the slide capture antigen off from the sample or standard.
  4. The end-user adds a detection mix containing paired antibodies (compatible with the primaries pre-coated on the slide) conjugated to a fluorescent dye for detection.
  5. Fluorescent signal from each spot is read using a laser slide scanner. The intensity from each spot is compared to the standard curve, and a quantitative expression profile for relevant biomarkers is established.
Restrictions For Research Use only
Handling Advice Do not touch the surface of the slides, as the microarray slides are very sensitive. Hold the slides by the edges only. Handle all buffers and slides with powder free gloves. Handle glass slide/s in clean environment. The Quantibody slides do not have bar codes. To help distinguish one slide from another, transcribe the slide serial number from the slide bag to the back of the slide with an ultra-fine point permanent marker. Please Note:Red permanent marker can significantly interfere with fluorescent signal detection. We recommend marking your slides with a green, blue or black ultra-fine point permanent marker. Please write the number on the very bottom edge of the slide. Do not write on the arrayed well areas.
Storage -20 °C
Storage Comment For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.